A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) test to Detect the Common Mutation (35delG) in the Connexin-26 Gene

Abstract

Objective: To develop a polymerase chain reaction (PCR) based test for the detection of a common frame-shift mutation (35delG) in the connexin-26 (GJB2) gene, and to investigate the status of this mutation in Oman. Method: A PCR test, based on sitedirected mutagenesis, was developed for the 35delG mutation. A mutagenesis primer generated an EcoN I site in a short (87 bp) DNA fragment amplifi ed from the connexin-26 gene. The EcoN I site is generated only if the 35delG mutation is present. Thus, a restriction fragment length polymorphism (RFLP) analysis of the amplifi ed DNA fragment with EcoN I allowed us to detect the 35delG mutation in the connexin 26 gene. Result: After validating the test using quality control DNA samples, which contained the 35delG mutation in either homozygous or heterozygous form, 120 healthy subjects and 35 unrelated Omani patients with nosyndromic autosomal recessive deafness (NARD), were screened for 35delG mutation. The mutation was not present in any individual tested. Conclusion: We have been able to develop a new PCR-RFLP test for detecting the 35delG common mutation in the connexin 26 gene. Our preliminary results from application of this test on a limited number of Omani patients indicate that the 35delG mutation may not be associated with NARD in Oman.

Keywords

• pcr-rflp, connexin-26 gene, 35delg mutation