COMPLETE GENOME SEQUENCE OF THE TYPE STRAIN BACTERIUM SPHAEROCHAETA ASSOCIATA GLS2T (VKM B-2742)T

Abstract

This study reports the complete genome sequence of Sphaerochaeta associata GLS2T (=VKM B-2742T =DSM 26261T), which was isolated from a consortium with methanogenic archaeon Methanosarcina mazei JL01. The consortium was collected from permafrost of the Kolyma lowland in Russia. The hybrid approach, combining paired-end Illumina reads with Oxford Nanopore Technologies MinION reads, was used to assemble the genome. The final assembly resulted in a circular chromosome that is 3,554,903 bp long. This high-quality genome assembly serves as a basis for algorithmic pathway reconstruction and postgenomic analysis. To further this research, the genome was imported into research portals for the algorithmic reconstruction of metabolic pathways, in both common sense (KEGG) and with special attention to carbohydrate metabolism (CAZy). These portals offer high-quality workplaces for in-depth studies.

Keywords

• геномное секвенирование
• sphaerochaeta
• гибридная геномная сборка
• genome sequencing
• sphaerochaeta
• hybrid genome assembly