In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

Panrao Liu; Yuncong Yin; Yabin Gong; Xusheng Qiu; Yingjie Sun; Lei Tan; Cuiping Song; Weiwei Liu; Ying Liao; Chunchun Meng; Chan Ding

doi:10.3390/v11100962

Viruses (Oct 2019)

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

Panrao Liu,
Yuncong Yin,
Yabin Gong,
Xusheng Qiu,
Yingjie Sun,
Lei Tan,
Cuiping Song,
Weiwei Liu,
Ying Liao,
Chunchun Meng,
Chan Ding

Affiliations

Panrao Liu: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Yuncong Yin: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China
Yabin Gong: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Xusheng Qiu: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Yingjie Sun: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Lei Tan: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Cuiping Song: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Weiwei Liu: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Ying Liao: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Chunchun Meng: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Chan Ding: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China

DOI: https://doi.org/10.3390/v11100962
Journal volume & issue: Vol. 11, no. 10
p. 962

Abstract

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Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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