Structural basis for TNA synthesis by an engineered TNA polymerase

Nicholas Chim; Changhua Shi; Sujay P. Sau; Ali Nikoomanzar; John C. Chaput

doi:10.1038/s41467-017-02014-0

Nature Communications (Nov 2017)

Structural basis for TNA synthesis by an engineered TNA polymerase

Nicholas Chim,
Changhua Shi,
Sujay P. Sau,
Ali Nikoomanzar,
John C. Chaput

Affiliations

Nicholas Chim: Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry University of California
Changhua Shi: Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry University of California
Sujay P. Sau: Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry University of California
Ali Nikoomanzar: Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry University of California
John C. Chaput: Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry University of California

DOI: https://doi.org/10.1038/s41467-017-02014-0
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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The laboratory-evolved polymerase Kod-RI catalyzes α-L-threose nucleic acid (TNA) synthesis. Here, the authors present Kod-RI crystal structures that give insights into how TNA triphosphates are selected and extended in a template-dependent manner, which will help to engineer improved TNA polymerases for synthetic genetics applications.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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