Cyclic Storage Chamber Ozonation as a Method to Inhibit Ethylene Generation during Plum Fruit Storage

Abstract

This study presents a method for inhibiting ethylene production during the room-temperature storage of plum fruits, using gaseous ozone (O3). The proposed storage strategy involves the cyclic ozone treatment of fruits every 24 h with specified O3 doses. Throughout the storage period, cyclic analyses of the atmosphere composition in storage chambers were conducted, measuring ethylene and carbon dioxide levels. Several parameters describing changes in fruit quality and biochemical transformations were systematically monitored until the end of the storage process. The results clearly indicate that fruits subjected to cyclic ozone treatment with the highest O3 doses during storage exhibit the slowest ripening rate. This reduced ripening rate is primarily attributed to the downregulation of S-adenosylmethionine synthetase expression, leading to a lower ethylene concentration in the storage chambers. Other obtained results concerning soluble solid content, titratable acidity, total polyphenols, anthocyanins, and vitamin C content confirm the observations regarding the impact of ozone treatment in slowing down the fruit ripening process. The best outcomes were achieved by applying a cyclic ozone process with a 100 ppm dose for 30 min.

Keywords

• <i>Prunus domestica</i>
• postharvest
• cold storage
• adenozyno-5′-trifosforan (ATP) biosynthesis of ethylene
• S-adenosulmethionine sythetase (SAMS)
• manganese superoxide dismutase (MnSOD)