Nature Communications (Apr 2020)
Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation
- Jai Woong Seo,
- Elizabeth S. Ingham,
- Lisa Mahakian,
- Spencer Tumbale,
- Bo Wu,
- Sadaf Aghevlian,
- Shahin Shams,
- Mo Baikoghli,
- Poorva Jain,
- Xiaozhe Ding,
- Nick Goeden,
- Tatyana Dobreva,
- Nicholas C. Flytzanis,
- Michael Chavez,
- Kratika Singhal,
- Ryan Leib,
- Michelle L. James,
- David J. Segal,
- R. Holland Cheng,
- Eduardo A. Silva,
- Viviana Gradinaru,
- Katherine W. Ferrara
Affiliations
- Jai Woong Seo
- Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University
- Elizabeth S. Ingham
- Department of Biomedical Engineering, University of California
- Lisa Mahakian
- Department of Biomedical Engineering, University of California
- Spencer Tumbale
- Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University
- Bo Wu
- Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University
- Sadaf Aghevlian
- Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University
- Shahin Shams
- Department of Biomedical Engineering, University of California
- Mo Baikoghli
- Department of Molecular and Cellular Biology, University of California
- Poorva Jain
- Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University
- Xiaozhe Ding
- Division of Biology and Biological Engineering, California Institute of Technology
- Nick Goeden
- Division of Biology and Biological Engineering, California Institute of Technology
- Tatyana Dobreva
- Division of Biology and Biological Engineering, California Institute of Technology
- Nicholas C. Flytzanis
- Division of Biology and Biological Engineering, California Institute of Technology
- Michael Chavez
- Department of Bioengineering, Stanford University
- Kratika Singhal
- Stanford University Mass Spectrometry
- Ryan Leib
- Stanford University Mass Spectrometry
- Michelle L. James
- Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University
- David J. Segal
- Genome Center and Department of Biochemistry and Molecular Medicine, University of California
- R. Holland Cheng
- Department of Molecular and Cellular Biology, University of California
- Eduardo A. Silva
- Department of Biomedical Engineering, University of California
- Viviana Gradinaru
- Division of Biology and Biological Engineering, California Institute of Technology
- Katherine W. Ferrara
- Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University
- DOI
- https://doi.org/10.1038/s41467-020-15818-4
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
Adeno-associated viruses (AAVs) can be targeted in a tissue-specific manner, but their tissue accumulation cannot be assessed in a non-invasive manner. Here the authors conjugate a multivalent chelator labelled with Cu-64 to the surface of AAVs and image the brain accumulation of the PHB.eB capsid by PET.
