Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food

Clara Tramuta; Lucia Decastelli; Francesco Ingravalle; Elisa Barcucci; Sandra Fragassi; Daniela Manila Bianchi

doi:10.3390/foods13040609

Foods (Feb 2024)

Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food

Clara Tramuta,
Lucia Decastelli,
Francesco Ingravalle,
Elisa Barcucci,
Sandra Fragassi,
Daniela Manila Bianchi

Affiliations

Clara Tramuta: Experimental Zooprophylactic Institute of Piedmont, Liguria and Valle d’Aosta, Italian National Reference Center for the Detection of Food Allergens and Substances Causing Food Intolerance (CReNaRiA), 10154 Turin, Italy
Lucia Decastelli: Experimental Zooprophylactic Institute of Piedmont, Liguria and Valle d’Aosta, Italian National Reference Center for the Detection of Food Allergens and Substances Causing Food Intolerance (CReNaRiA), 10154 Turin, Italy
Francesco Ingravalle: Experimental Zooprophylactic Institute of Piedmont, Liguria and Valle d’Aosta, Department Biostatistics, Epidemiology and Risk Analysis (BEAR), 10154 Turin, Italy
Elisa Barcucci: Experimental Zooprophylactic Institute of Piedmont, Liguria and Valle d’Aosta, Italian National Reference Center for the Detection of Food Allergens and Substances Causing Food Intolerance (CReNaRiA), 10154 Turin, Italy
Sandra Fragassi: Experimental Zooprophylactic Institute of Piedmont, Liguria and Valle d’Aosta, Italian National Reference Center for the Detection of Food Allergens and Substances Causing Food Intolerance (CReNaRiA), 10154 Turin, Italy
Daniela Manila Bianchi: Experimental Zooprophylactic Institute of Piedmont, Liguria and Valle d’Aosta, Italian National Reference Center for the Detection of Food Allergens and Substances Causing Food Intolerance (CReNaRiA), 10154 Turin, Italy

DOI: https://doi.org/10.3390/foods13040609
Journal volume & issue: Vol. 13, no. 4
p. 609

Abstract

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In accordance with U.S. FDA Foods Program Regulatory Science Steering Committee guidelines, with this study, we optimized and validated a commercial real-time PCR method for the detection of low amounts of lupin in four classes of food matrices: chocolate cookies, ragù, Olivier salad, and barley and rice flour. DNA extracted from blank (true negative) samples artificially contaminated with lupin (Lupinus albus) flour at 1000 ppm underwent dilutions with the DNA extracted from the true negative samples up to 0.5 ppm. The limit of detection for real-time PCR was 0.5 ppm in the complex matrices (range, Ct 26–34), making this a specific, robust, and rapid method for lupin allergen detection and labeling. Our validation data support the suitability of this commercially available real-time PCR method for this purpose.

Published in Foods

ISSN: 2304-8158 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/foods

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