Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Stephan Riesenberg; Nelly Helmbrecht; Philipp Kanis; Tomislav Maricic; Svante Pääbo

doi:10.1038/s41467-022-28137-7

Nature Communications (Jan 2022)

Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Stephan Riesenberg,
Nelly Helmbrecht,
Philipp Kanis,
Tomislav Maricic,
Svante Pääbo

Affiliations

Stephan Riesenberg: Max Planck Institute for Evolutionary Anthropology
Nelly Helmbrecht: Max Planck Institute for Evolutionary Anthropology
Philipp Kanis: Max Planck Institute for Evolutionary Anthropology
Tomislav Maricic: Max Planck Institute for Evolutionary Anthropology
Svante Pääbo: Max Planck Institute for Evolutionary Anthropology

DOI: https://doi.org/10.1038/s41467-022-28137-7
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 8

Abstract

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Some DNA sequences are refractory to CRISPR-Cas9 cleavage, partially due to gRNA misfolding. Here the authors engineer gRNAs to prevent misfolding and further enhanced their stability by chemical modifications allowing robust genome editing regardless of target sequence.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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