Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors

Abstract

Purpose: to compare the regenerative activity of benign and malignant conjunctival tumors.Material and methods. The study was performed on 86 tumor biopsies from patients with a clinically diagnosed conjunctival neoplasm. Healthy tissues from the same eye were taken as a control sample. The optical density of the reaction mixture containing biogenic nanoparticles formed in the presence of conjunctival tumors and the corresponding paired healthy tissue samples from the control group was measured by spectrophotometry. The data obtained were later verified by a pathohistological analysis.Results. A significantly higher level of in situ formation of biogenic silver nanoparticles was recorded in the reaction mixture with malignant tumors of the conjunctiva (3.0 ± 1.1, (n = 32) compared to benign tumors (1.3 ± 0.2, n = 54). No differences were found between various types of benign tumors. In samples of malignant tumors, the indicators of recovery activity in melanoma (3.4 ± 1.0, n = 14) and lymphoma (2.8 ± 1.0, n = 7) were significantly higher than in squamous cell carcinoma (2.0 ± 0.6, n = 11), but no significant differences were found between the two types of tumors.Conclusion. The method of spectrophotometric measurement of the regenerative activity of conjunctival tumors can be used in preoperative or intraoperative diagnostics thanks to the fact that the results can be obtained rapidly, which will help to quickly determine the extent of surgical intervention needed and optimize the treatment tactics.

Keywords

• tumor
• conjunctiva
• biogenic nanoparticles
• spectrophotometry