Investigation of the Effect of High-Intensity Interval Training (HIIT) on the Expression of the Genes miR-126, miR-296, HGS and VEGF-A Protein Levels in Tumor Tissue in Female Mice

Abstract

Background & Objective: The studies of the last two decades have shown that regular training is associated with the reduction of mortality among patients with breast cancer, and has an important role in inhibiting breast cancer progression as well as treatment of the disease. However, the micro-molecular mechanisms involved are not fully understood yet. However, the aim of the present study was to investigate the effect of a high-intensity interval training (HIIT) on the expression of miR-126, miR-296, HGS gene, and VEGF-A protein level in tumor tissue of the mice with breast cancer. Materials & Methods: For this purpose, 12 BALB/c mice (6-8 weeks, weight 19 ± 1.05 g), after induction of cancer (MC4-L2 subcutaneous injection into the right side of the mice), were randomly divided into two groups with 6 in each group: HIIT and control. Each session of HIIT group program includes six intervals of three minutes and 20 seconds with an intensity of 85 to 90 percent of VO2max and 1-minute recovery with 30 to 35 percent VO2max intensity between each interval; and the animals were sacrificed 24 hours after the last training session and the expression of miR-126, miR-296, HBS gene and VEGF protein levels in tumor tissues were examined. Results: The statistical results showed that the implementation of HIIT significantly reduced the expression of miR-296 and subsequently increased the HGS gene expression and led to increased expression levels of miR-126 and decreased levels of VEGF-A protein in the training group compared to the control group (p<0.05). Conclusion: It seems that HIIT, by suppressing tumor angiogenesis signaling pathways, could be an effective intervention to inhibit the growth of breast tumors.

Keywords

• breast cancer
• exercise training
• mir-126
• mir-296
• vegf-a