Proteomic analysis of a Candida albicans pga1 Null Strain

Andy Awad; Pamela El Khoury; Brigitte Wex; Roy A. Khalaf

EuPA Open Proteomics (Jun 2018)

Proteomic analysis of a Candida albicans pga1 Null Strain

Andy Awad,
Pamela El Khoury,
Brigitte Wex,
Roy A. Khalaf

Affiliations

Andy Awad: Department of Natural Sciences, Lebanese American University, PO Box 36, Byblos, Lebanon
Pamela El Khoury: Department of Natural Sciences, Lebanese American University, PO Box 36, Byblos, Lebanon
Brigitte Wex: Department of Natural Sciences, Lebanese American University, PO Box 36, Byblos, Lebanon
Roy A. Khalaf: Corresponding author.; Department of Natural Sciences, Lebanese American University, PO Box 36, Byblos, Lebanon

Journal volume & issue: Vol. 18
pp. 1 – 6

Abstract

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We previously characterized Pga1, a Candida albicans (C. albicans) cell wall protein necessary for proper virulence, adhesion, and resistance to oxidative stress. By utilizing tandem mass spectrometry coupled with bioinformatics to investigate cell wall proteome expression in a pga1 null fourteen and 36 proteins were identified in the wild type grown under filamentous and non-filamentous conditions respectively, but were not detected in the mutant, including members of the PGA GPI anchored family. Virulence and adhesion proteins such as Hsp 90, Sap10, Cdc11, Int 3 and members of the lipase family were also identified exclusively in the wild type. Keywords: Candida albicans, Proteomics, Cell wall, MALDI, MS/MS

Published in EuPA Open Proteomics

ISSN: 2212-9685 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General): Genetics
Website: http://www.journals.elsevier.com/eupa-open-proteomics/

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