Data on Drosophila clots and hemocyte morphologies using GFP-tagged secretory proteins: Prophenoloxidase and transglutaminase

Alexis Dziedziech; Martin Schmid; Badrul Arefin; Thomas Kienzle; Robert Krautz; Ulrich Theopold

Data in Brief (Aug 2019)

Data on Drosophila clots and hemocyte morphologies using GFP-tagged secretory proteins: Prophenoloxidase and transglutaminase

Alexis Dziedziech,
Martin Schmid,
Badrul Arefin,
Thomas Kienzle,
Robert Krautz,
Ulrich Theopold

Affiliations

Alexis Dziedziech: Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden
Martin Schmid: Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden
Badrul Arefin: Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden
Thomas Kienzle: Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden
Robert Krautz: Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden
Ulrich Theopold: Corresponding author.; Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden

Journal volume & issue: Vol. 25

Abstract

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Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors Schmid et al., 2019. The linked article demonstrates the localization of two secretory proteins in Drosophila melanogaster, Prophenoloxidase (PPO2) and Transglutaminase-A (Tg) in hemocytes as well the clot with different tissue-specific drivers. Here we provide further data for the usefulness of the GFP-tagged version of the two crosslinking enzymes that are involved in clot hardening. The morphology of crystal cells is described using GFP-tagged PPO2 rather than with the use of antibodies in ex vivo hemolymph preparations. The use of the GFP-tagged proteins PPO2 and Tg is shown in additional contexts. Keywords: Insect immunity, Innate immunity, Non-classical secretion, Transglutaminase, Prophenoloxidase, Coagulation, Hemocytes

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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