Data on charge separation of bispecific and mispaired IgGs using native charge-variant mass spectrometry

Abstract

The data supplied in this work are related to the research article entitled “Characterization of Bispecific and Mispaired IgGs by Native Charge-Variant Mass Spectrometry” (Phung et al., 2019). This data article describes a powerful analytical platform using native weak cation exchange chromatography coupled to a high-resolution mass spectrometer, charge variant mass spectrometry (CV-MS), to characterize bispecific and mispaired antibody species. Elution order is investigated through analytical methods and molecular modeling in an effort to understand the intrinsic charge, size and shape differences of these molecules.

Keywords

• Bispecific antibody
• Cognate pairs
• Single cell
• Charge variant mass spectrometry
• Ion exchange chromatography
• Weak cation exchange