Fabrication and Characterization of Nanofibrous Wound Dressings Containing Gentamicin-conjugated Nanoclay

Abstract

Introduction: The development of bioactive wound dressings with high healing efficacy is one of the most important domains in interdisciplinary fields. The objective of the present study was to fabricate and characterize a nanofibrous wound dressing containing gentamicin-conjugated nanoclay. Material & Methods: First, the interaction between the structure of nanoclays and gentamicin was evaluated based on computer simulation methods and using Gaussian software. Then, the purified nanoclays were conjugated with gentamicin, and the properties of the resulting nanoparticles were analyzed. The synthesized nanocomplex was integrated into the nanofiber structure using the electrospinning method, and the nanofiber wound covering was identified. Finally, the biological properties of nanofiber wound dressing were investigated. Results: Numerical studies indicated that there were negatively charged oxygen groups on both sides of the nanoclay plates, which could make stable interactions with the positively charged nitrogen in gentamicin, and the drug was well-loaded between pages. The characterization results demonstrated that nanofiber wound dressings had acceptable characteristics in terms of morphology, diameter, wettability, mechanical properties, swelling, and bacterial penetration barrier. These functions provide a suitable environment for wound healing. The addition of gentamicin led to beneficial biological properties for prepared wound dressings, and these nanofibers had acceptable hemocompatibility and biocompatibility. Discussion & Conclusion: The findings of the present research can be a fundamental step for using the combination of kaolin/polyvinyl alcohol nanofibers containing gentamicin in various fields of pharmaceutical bioengineering, including the production of wound dressings.

Keywords

• nanofiber wound dressing
• gentamycin
• polyvinyl alcohol (pva)
• kaolin
• nanoclay
• gossin