A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

Ricardo Felipe Alexandre de Mello; Wandson B. de Souza Pinheiro; Jaisielle Kelem F. Benjamim; Francilia Campos de Siqueira; Renan Campos Chisté; Alberdan Silva Santos

Arabian Journal of Chemistry (Aug 2021)

A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

Ricardo Felipe Alexandre de Mello,
Wandson B. de Souza Pinheiro,
Jaisielle Kelem F. Benjamim,
Francilia Campos de Siqueira,
Renan Campos Chisté,
Alberdan Silva Santos

Affiliations

Ricardo Felipe Alexandre de Mello: Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil; Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil; Corresponding authors at: Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil.
Wandson B. de Souza Pinheiro: Faculty of Chemical Engineering, Institute of Technology, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil
Jaisielle Kelem F. Benjamim: Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil; Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil; Corresponding authors at: Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil.
Francilia Campos de Siqueira: Faculty of Food Engineering, Institute of Technology, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil
Renan Campos Chisté: Faculty of Food Engineering, Institute of Technology, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil
Alberdan Silva Santos: Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil; Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil; Corresponding authors at: Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity, Institute of Exact and Natural Sciences, Federal University of Pará, 01 Augusto Corrêa Street, Guamá, ZIP Code 66075-110 Belém, Pará, Brazil.

Journal volume & issue: Vol. 14, no. 8
p. 103252

Abstract

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In this work, we present a preparative elution method by high-speed countercurrent chromatography (Prep-HSCCC) for the separation and isolation of the main bioactive xanthones present in the alcoholic extract of Garcinia mangostana L. agro-industrial waste. The method proved to be quite efficient, operating with a two-phase solvent system consisting of Methanol/water/Ethanol/Hexane/methyl tert-butyl ether (6:3:1:6:4v/v), tail-head elution mode, with mobile phase flow of 5 mL/min and rotation speed of 800 rpm. The method demonstrated repeatability (RSD = 3.8–6.5%) and allowed the isolation of α-mangostin and γ-mangostin xanthones, both with purities above 93%, in a single step, performed in a time of 35 min. Isolated substances were identified by HPLC-PDA-MS and 1H and 13C NMR spectroscopy. Both xanthones were efficient scavengers of DPPH·· and ABTS·+ radicals in a concentration-dependent manner with EC50 at 8.43 and 3.31 µg/mL, respectively, for γ-mangostin, and 71.10 and 29.33 µg/mL, respectively, for α-mangostin.

Published in Arabian Journal of Chemistry

ISSN: 1878-5352 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: http://www.journals.elsevier.com/arabian-journal-of-chemistry/

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