Taxonomic composition of microbiota of colon in breastfed infants with acute colienteritis

Abstract

Introduction: In recent years, paradoxical situation has been created, that testifies adverse evolution of modern acute intestinal infections, especially in infants and vital prognosis for patients by measure of deep study of this disease in patients, which number is significant and continues to grow, and the prognosis is getting worse. Aim: To define the etiology of colienteritis in infants (1–6 months old), the taxonomic composition of pathogenic and conditionally pathogenic microorganisms. Materials and methods: Content of colon of 48 children (one to six months old) with colienteritis underwent bacterial and mycological examination (control group – 35 samples of colon content of practically healthy infants). Results: Etiological structure was determined in 28 (58,33 %) of investigations. Consistency index, frequency of occurrence, Margalef species richness, Whittaker species diversity, Simpson and Berger–Parker species dominance indices of bacteria of genera Bifidobacterium, Lactobacillus, Bacteroides and Escherichia did not differ in patients and healthy children. These indices grow in Peptostreptococci: constancy index – by 78,26 %, frequency of occurrence – by 60,00 %, Margalef species richness index – by 2 times, Whittaker species diversity index – by 97,32 %, Simpson species dominance index – by 3 times and Berger - Parker index – by 65,31 %. These indices also grew in conditionally pathogenic Enterobacteria (Proteus) by 82,24 %, by 2 times, by 2,03 times, by 68,18 % respectively. Study of taxonomic composition of colon microbiota in children with acute colienteritis showed widespread contamination of biotope (cavity) by pathogenic (E. coli Hly +, enteropathogenic E. coli) and conditionally pathogenic (C. diversus, Proteus ssp.) Enterobacteria, Staphylococci, Peptococcus. This is accompanied with elimination of bacteria of genus Eubacterium from colon cavity. Conclusions: Acute colienteritis in one to six months old breastfed children developed on the background of reducing value of Bifidobacterium and Lactobacillus and the contamination of colon with E. coli Hly+, enteropathogenic Escherichia and C. diversus, Proteus, Peptococi, Staphylococci and Peptostreptococci, on the background of strengthening of value of Bacteroides and Escherichia.

Keywords

• infants (1–6 months)
• bacterial infections
• intestine large
• microbiota