Using an inexpensive module for Quantitative Phase Imaging

Abstract

We report on modular implementation of digital holography that we recently proposed. The module is designed such that it may be added to an existing brightfield microscope’s image port for digital holographic microscopy functionality. The proposed system is modular, portable, and cost-effective and not require path-length realignment when changing samples. The square in-line Mach-Zender architecture is used and the off-axis condition is achieved using two sets of wedge prism pairs; this approach offers advantages over other Mach-Zender nearly common-path modules, particularly in path length matching of object and reference wavefields for low-temporal coherence sources. Additionally, the proposed system allows for continuous variation of the tilt angles of the object and reference wavefields incident on the sensor, making it readily adaptable to any microscope and camera.