A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Alisha House; Erica Fatica; Rohan Shah; Jared Stergar; Ryan Pearce; Yana Sandlers

MethodsX (Jan 2020)

A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Alisha House,
Erica Fatica,
Rohan Shah,
Jared Stergar,
Ryan Pearce,
Yana Sandlers

Affiliations

Alisha House: Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States
Erica Fatica: Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States
Rohan Shah: Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States
Jared Stergar: Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States
Ryan Pearce: Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States
Yana Sandlers: Corresponding author.; Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States

Journal volume & issue: Vol. 7
p. 100572

Abstract

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Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

Published in MethodsX

ISSN: 2215-0161 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: http://www.journals.elsevier.com/methodsx/

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