Inhalation of Atmospheric-Pressure Gas Plasma Attenuates Brain Infarction in Rats With Experimental Ischemic Stroke

Ye Chen; Ye Chen; Bingyan Yang; Lixin Xu; Zhongfang Shi; Ruoyu Han; Fang Yuan; Jiting Ouyang; Xu Yan; Kostya Ken Ostrikov

doi:10.3389/fnins.2022.875053

Frontiers in Neuroscience (Apr 2022)

Inhalation of Atmospheric-Pressure Gas Plasma Attenuates Brain Infarction in Rats With Experimental Ischemic Stroke

Ye Chen,
Ye Chen,
Bingyan Yang,
Lixin Xu,
Zhongfang Shi,
Ruoyu Han,
Fang Yuan,
Jiting Ouyang,
Xu Yan,
Kostya Ken Ostrikov

Affiliations

Ye Chen: Department of Pathophysiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
Ye Chen: School of Basic Medical Sciences, Capital Medical University, Beijing, China
Bingyan Yang: School of Physics, Beijing Institute of Technology, Beijing, China
Lixin Xu: Department of Pathophysiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
Zhongfang Shi: Department of Pathophysiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
Ruoyu Han: School of Physics, Beijing Institute of Technology, Beijing, China
Fang Yuan: Department of Pathophysiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
Jiting Ouyang: School of Physics, Beijing Institute of Technology, Beijing, China
Xu Yan: Department of Pathophysiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
Kostya Ken Ostrikov: School of Chemistry and Physics and Centre for Biomedical Technologies, Queensland University of Technology, Brisbane, QLD, Australia

DOI: https://doi.org/10.3389/fnins.2022.875053
Journal volume & issue: Vol. 16

Abstract

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Previous studies suggest the potential efficacy of neuroprotective effects of gaseous atmospheric-pressure plasma (APP) treatment on neuronal cells. However, it remains unclear if the neuroprotective properties of the gas plasmas benefit the ischemic stroke treatment, and how to use the plasmas in the in vivo ischemic stroke models. Rats were subjected to 90 min middle cerebral artery occlusion (MCAO) to establish the ischemic stroke model and then intermittently inhaled the plasma for 2 min at 60 min MCAO. The regional cerebral blood flow (CBF) was monitored. Animal behavior scoring, magnetic resonance imaging (MRI), 2,3,5-triphenyltetrazolium chloride (TTC) staining, and hematoxylin and eosin (HE) staining were performed to evaluate the therapeutic efficacy of the gas plasma inhalation on MCAO rats. Intermittent gas plasma inhalation by rats with experimental ischemic stroke could improve neurological function, increase regional CBF, and decrease brain infarction. Further MRI tests showed that the gas plasma inhalation could limit the ischemic lesion progression, which was beneficial to improve the outcomes of the MCAO rats. Post-stroke treatment with intermittent gas plasma inhalation could reduce the ischemic lesion progression and decrease cerebral infarction volume, which might provide a new promising strategy for ischemic stroke treatment.

Published in Frontiers in Neuroscience

ISSN: 1662-4548 (Print); 1662-453X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: http://www.frontiersin.org/neuroscience

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