Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis

Abstract

Yucca schidigera Roezl (Mojave), a kind of ornamental plant belonging to the Yucca genus (Agavaceae), whose extract exhibits important roles in food, beverage, cosmetic and feed additives owing to its rich spirostanol saponins. To provide a comprehensive chemical profiling of the spirostanol saponins in it, this study was performed by using a multi-phase liquid chromatography method combining a reversed phase chromatography T3 column with a normal phase chromatography silica column for the separation and an ESI-Q-Exactive-Orbitrap MS in positive ion mode as the detector. By comparing the retention time and ion fragments with standards, thirty-one spirostanol saponins were identified. In addition, according to the summary of the chromatographic retention behaviors and the MS/MS cleavage patterns and biosynthetic pathway, another seventy-nine spirostanol saponins were speculatively identified, forty ones of which were potentially new ones. Moreover, ten novel spirostanol saponins (three pairs of (25R/S)-spirostanol saponin isomer mixtures) were targeted for isolation to verify the speculation. Then, the comprehensive chemical profiling of spirostanol saponins from Y. schidigera was reported here firstly.

Keywords

• <i>Yucca schidigera Roezl</i> (Mojave)
• spirostanol saponins
• chemical constituents profiling
• multi-phase liquid chromatography
• normal phase chromatography silica column separation
• reversed phase liquid chromatography/mass spectrometry analysis