Insulated transcriptional elements enable precise design of genetic circuits

Yeqing Zong; Haoqian M. Zhang; Cheng Lyu; Xiangyu Ji; Junran Hou; Xian Guo; Qi Ouyang; Chunbo Lou

doi:10.1038/s41467-017-00063-z

Nature Communications (Jul 2017)

Insulated transcriptional elements enable precise design of genetic circuits

Yeqing Zong,
Haoqian M. Zhang,
Cheng Lyu,
Xiangyu Ji,
Junran Hou,
Xian Guo,
Qi Ouyang,
Chunbo Lou

Affiliations

Yeqing Zong: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
Haoqian M. Zhang: Peking-Tsinghua Joint Center for Life Sciences, Peking University
Cheng Lyu: School of Physics, Peking University
Xiangyu Ji: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
Junran Hou: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
Xian Guo: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
Qi Ouyang: Peking-Tsinghua Joint Center for Life Sciences, Peking University
Chunbo Lou: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s41467-017-00063-z
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 13

Abstract

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Unwanted interactions between cellular components can complicate rational engineering of biological systems. Here the authors design insulated minimal promoters and operators that enable biophysical modeling of bacterial transcription without free parameters for precise circuit design.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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