Engineered dual selection for directed evolution of SpCas9 PAM specificity

Gregory W. Goldberg; Jeffrey M. Spencer; David O. Giganti; Brendan R. Camellato; Neta Agmon; David M. Ichikawa; Jef D. Boeke; Marcus B. Noyes

doi:10.1038/s41467-020-20650-x

Nature Communications (Jan 2021)

Engineered dual selection for directed evolution of SpCas9 PAM specificity

Gregory W. Goldberg,
Jeffrey M. Spencer,
David O. Giganti,
Brendan R. Camellato,
Neta Agmon,
David M. Ichikawa,
Jef D. Boeke,
Marcus B. Noyes

Affiliations

Gregory W. Goldberg: Institute for Systems Genetics, NYU Langone Health
Jeffrey M. Spencer: Institute for Systems Genetics, NYU Langone Health
David O. Giganti: Institute for Systems Genetics, NYU Langone Health
Brendan R. Camellato: Institute for Systems Genetics, NYU Langone Health
Neta Agmon: Institute for Systems Genetics, NYU Langone Health
David M. Ichikawa: Institute for Systems Genetics, NYU Langone Health
Jef D. Boeke: Institute for Systems Genetics, NYU Langone Health
Marcus B. Noyes: Institute for Systems Genetics, NYU Langone Health

DOI: https://doi.org/10.1038/s41467-020-20650-x
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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The PAM specificity of SpCas9 can be altered with positive selection during directed evolution. Here the authors use simultaneous positive and negative selection to improve activity on NAG PAMs while reducing activity on NGG PAMs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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