Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1

Abstract

Objective To investigate how oxaliplatin regulates the MAPK pathway and inhibits the proliferation of gastric cancer cells. Methods From NCBI, TargetScan, StarBase and miRBase databases were used to perform GO analysis and KEGG pathway enrichment in DAVID analysis, to find related miRNAs and predict their target genes. The proliferation, cell cycle, invasion and protein expression of SGC-7901 cells were analyzed by Real-time PCR, MTT, Hoechst33258, flow cytometry, scratch assay and Western blot, respectively. Results miR-7-5p expression was significantly downregulated in gastric cancer cells. RAF1 and miR-7-5p were co-targeted. The apoptosis of SGC-7901 cells was promoted, the percentage of G1 phase cells and the expression of caspase3 and caspase9 were increased, while the percentage of G2 phase cells and the ratio of Bcl-2/Bax protein were decreased in miR-7-5p mimics and oxaliplatin (OXA) groups(P < 0.05). Conclusion Both overexpression of miR-7-5p and oxaliplatin could promote the apoptosis of gastric cancer SGC-7901 cells, suggesting that oxaliplatin may promote the apoptosis of SGC-7901 cells and reduce the rates of invasion and migration by up-regulating miRNA-7-5p.

Keywords

• mir-7-5p
• oxaliplatin
• gastric cancer
• apoptosis
• invasion