Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

Manuel C. Martos-Maldonado; Christian T. Hjuler; Kasper K. Sørensen; Mikkel B. Thygesen; Jakob E. Rasmussen; Klaus Villadsen; Søren R. Midtgaard; Stefan Kol; Sanne Schoffelen; Knud J. Jensen

doi:10.1038/s41467-018-05695-3

Nature Communications (Aug 2018)

Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

Manuel C. Martos-Maldonado,
Christian T. Hjuler,
Kasper K. Sørensen,
Mikkel B. Thygesen,
Jakob E. Rasmussen,
Klaus Villadsen,
Søren R. Midtgaard,
Stefan Kol,
Sanne Schoffelen,
Knud J. Jensen

Affiliations

Manuel C. Martos-Maldonado: Department of Chemistry, University of Copenhagen
Christian T. Hjuler: Department of Chemistry, University of Copenhagen
Kasper K. Sørensen: Department of Chemistry, University of Copenhagen
Mikkel B. Thygesen: Department of Chemistry, University of Copenhagen
Jakob E. Rasmussen: Department of Chemistry, University of Copenhagen
Klaus Villadsen: Department of Chemistry, University of Copenhagen
Søren R. Midtgaard: Niels Bohr Institute, University of Copenhagen
Stefan Kol: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
Sanne Schoffelen: Department of Chemistry, University of Copenhagen
Knud J. Jensen: Department of Chemistry, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-018-05695-3
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

Read online

His-tagged proteins can undergo N-terminal acylation as an undesired side-reaction. Here, the authors utilize this to develop a method for highly selective acylation and further modification of peptides and proteins using an optimized His sequence and 4-methoxyphenyl esters as acyl donors.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal