TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells

Abstract

Shao et al. develop an imaging probe, TagBiFC, to visualize protein-protein interaction in living cells with better signal-to-noise ratio and photostability than fluorescent proteins. This probe provides better localization and longer tracking time and authors visualise transcription factor dimerization, single-molecule nucleosome, and background-free mRNA imaging using TagBiFC.