CdS Nanoparticle-based Biosensor Development for Aflatoxin Determination

Abstract

An anodic stripping voltammetry detection method with lateral flow immunochromatographic assay for determining aflatoxin M1 was developed. Cadmium sulfide nanoparticles (CdSNPs) were employed as a label for aflatoxin antibody. Conjugation between the CdSNPs and aflatoxin antibody was performed as the first step. The CdSNP-antibody conjugate was then immobilized on a test strip on a conjugate pad. When the conjugate interacted with the sample containing aflatoxin, it was captured by the aflatoxin in the test zone. The CdSNPs retained in the test zone were then measured by anodic stripping voltammetry using a boron-doped diamond electrode. A linear concentration range of 0–70 ppb aflatoxin, with a sensitivity of 20 µA/mM and a detection limit of 30 ppb, was achieved by this method.

Keywords

• Aflatoxin
• Anodic stripping voltammetry
• Boron-doped diamond
• CdS nanoparticles