Dexmedetomidine attenuates anoxia- induced injury of human neuroblastoma cell line SH-SY5Y

Abstract

Objective To study the protective effect of dexmedetomidine (DEX) on human neuroblastoma cell line SH-SY5Y under anoxia condition and its specific mechanism. Methods SH-SY5Y cells were divided into control group, anoxia group (treated with final concentration of 1.2 mmol/L CoCl2 for 24 h) and DEX intervention group (treated with final concentration of 1.2 mmol/L CoCl2 and 10 μmol/L DEX for 24 h). Cell morphology was observed by microscope; Cell viability and apoptosis were detected by MTT assay, TUNEL staining and annexin V-FITC/PI staining; The expression levels of cleaved caspase-9, cleaved caspase-3, p75NTR and p-NF-κB proteins were detected by Western blot. Results Compared with control group, the cell size in anoxia group was shrinked(PPPNTR and p-NF-κB proteins was all significantly increased (PPConclusions Alleviation of anoxia-induced SH-SY5Y cell injury is potentially mediatd by inhibiting p75NTR expression and NF-κB activity.

Keywords

• dexmedetomidine|sh-sy5y cell|p75ntr|anoxia