Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Min Liu; Ning Ge; Yongfeng Han; Jamie Reilly; Meimei Yang; Fan Yang; Janusz Krawczyk; Veronica McInerney; Timothy O'Brien; Terence Prendiville; Sanbing Shen

Stem Cell Research (Dec 2021)

Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Min Liu,
Ning Ge,
Yongfeng Han,
Jamie Reilly,
Meimei Yang,
Fan Yang,
Janusz Krawczyk,
Veronica McInerney,
Timothy O'Brien,
Terence Prendiville,
Sanbing Shen

Affiliations

Min Liu: Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, China
Ning Ge: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland
Yongfeng Han: Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, China
Jamie Reilly: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland
Meimei Yang: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland
Fan Yang: Hebei Province Children's Hospital, Shijiazhuang, Hebei, China
Janusz Krawczyk: Department of Haematology, Galway University Hospital, Ireland
Veronica McInerney: HRB Clinical Research Facility, National University of Ireland (NUI) Galway, Ireland
Timothy O'Brien: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland; Corresponding authors at: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland (S. Shen).
Terence Prendiville: National Children's Research Centre, Children's Health Ireland at Crumlin, Dublin 12, Ireland; Corresponding authors at: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland (S. Shen).
Sanbing Shen: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland; Corresponding authors at: Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland (S. Shen).

Journal volume & issue: Vol. 57
p. 102607

Abstract

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Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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