Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds

Míriam Silva Rafael; Leticia Cegatti Bridi; Igor V. Sharakhov; Osvaldo Marinotti; Maria V. Sharakhova; Vladimir Timoshevskiy; Giselle Moura Guimarães-Marques; Valéria Silva Santos; Carlos Gustavo Nunes da Silva; Spartaco Astolfi-Filho; Wanderli Pedro Tadei

doi:10.3390/insects12020164

Insects (Feb 2021)

Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds

Míriam Silva Rafael,
Leticia Cegatti Bridi,
Igor V. Sharakhov,
Osvaldo Marinotti,
Maria V. Sharakhova,
Vladimir Timoshevskiy,
Giselle Moura Guimarães-Marques,
Valéria Silva Santos,
Carlos Gustavo Nunes da Silva,
Spartaco Astolfi-Filho,
Wanderli Pedro Tadei

Affiliations

Míriam Silva Rafael: Coordenação de Sociedade Ambiente e Saúde, Laboratório de Vetores de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Manaus, AM 69060-001, Brazil
Leticia Cegatti Bridi: Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, Brazil
Igor V. Sharakhov: Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Osvaldo Marinotti: MTEKPrime, Aliso Viejo, CA 92656, USA
Maria V. Sharakhova: Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Vladimir Timoshevskiy: Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Giselle Moura Guimarães-Marques: Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, Brazil
Valéria Silva Santos: Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, Brazil
Carlos Gustavo Nunes da Silva: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas, Av. Rodrigo Otávio, 6.200. Coroado l, Manaus, AM 69080-900, Brazil
Spartaco Astolfi-Filho: Laboratorio de Tecnologias de DNA, Divisão de Biotecnologia, Centro de Apoio Multidisciplinar, Universi dade Federal do Amazonas, Av. Rodrigo Otávio, 6.200. Coroado l, Manaus, AM 69080-900, Brazil
Wanderli Pedro Tadei: Coordenação de Sociedade Ambiente e Saúde, Laboratório de Vetores de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Manaus, AM 69060-001, Brazil

DOI: https://doi.org/10.3390/insects12020164
Journal volume & issue: Vol. 12, no. 2
p. 164

Abstract

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The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the An. darlingi scaffolds with homologous sequences of the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.

Published in Insects

ISSN: 2075-4450 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science
Website: http://www.mdpi.com/journal/insects/

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