Determination_of D003 by capillary gas chromatography

Abstract

D003 is a mixture of saturated very long-chain fatty acids ranging from 24 to 36 carbon atoms, obtained from sugar cane (Saccharum officinarum L.) wax, with antiplatelet, 'antithrombotic, antioxidant and cholesterol-lowering effects . A capillary gas chromatographic method for the determination of this product was developed and validated . The acids were analyzed as methyl ester derivatives and separated in a BP-5 widebore column using 1-nonacosanoic acid as internal standard . A good linearity (r > 0 .99; CVs of the response factors and of the slopes lower than 5 and 2 %, respectively) ; as well as absence of bias was found for both, total and individual acids in the whole studied concentration range, from 30 to 150 % of the nominal mass . Quantitative recoveries (98 .9 and 100 .62 %) and good intermediate precision (CV = 0 .96 %) were also obtained . The specificity study showed no interferences regarding the determination of this mixture, once the samples were submitted to stress conditions. According to these results, it can be ensure that the validated procedure is appropriate for the quality control and stability studies of this product.