Screening, identification, optimization of fermentation conditions, and extraction of secondary metabolites for the biocontrol of Rhizoctonia Solani AG-3

Abstract

In this study a strain of Streptomyces sp. was isolated from soil and identified by 16S rRNA gene sequencing technology. The strain was screened for antibiotics production effective against biocontrol of Rhizoctonia solani AG-3 to cure the target spot disease in tobacco. For enhance production of secondary metabolites, central composite design of response surface methodology (RSM) was applied in submerged fermentation. The maximum metabolite production was using medium volume of 55 mL in 250 mL flask, agitation speed of 165 rpm, incubation temperature 30 °C, initial medium pH of 6.8 and inoculum size of 7%. Solvent extraction method was used to extract the secondary metabolites and active compounds were purified by silica gel column chromatography. The purified fractions were further investigated by gas chromatography-mass spectrophotometer (GC-MS). GC-MS analysis showed 48 compounds, among them 12 were active against pathogen. These findings indicated that the strain Streptomyces TA 1123 was a potential antagonist against R. solani AG-3.

Keywords

• Streptomyces
• biocontrol
• Rhizoctonia solani
• RSM