Frontiers in Physiology (Jan 2020)
CRISPR/Cas9-Mediated Vitellogenin Receptor Knockout Leads to Functional Deficiency in the Reproductive Development of Plutella xylostella
- Lu Peng,
- Lu Peng,
- Lu Peng,
- Lu Peng,
- Qing Wang,
- Qing Wang,
- Qing Wang,
- Qing Wang,
- Ming-Min Zou,
- Ming-Min Zou,
- Ming-Min Zou,
- Ming-Min Zou,
- Yu-Dong Qin,
- Yu-Dong Qin,
- Yu-Dong Qin,
- Yu-Dong Qin,
- Liette Vasseur,
- Liette Vasseur,
- Liette Vasseur,
- Liette Vasseur,
- Liette Vasseur,
- Li-Na Chu,
- Li-Na Chu,
- Li-Na Chu,
- Li-Na Chu,
- Yi-Long Zhai,
- Yi-Long Zhai,
- Yi-Long Zhai,
- Yi-Long Zhai,
- Shi-Jie Dong,
- Shi-Jie Dong,
- Shi-Jie Dong,
- Shi-Jie Dong,
- Li-Li Liu,
- Li-Li Liu,
- Li-Li Liu,
- Li-Li Liu,
- Wei-Yi He,
- Wei-Yi He,
- Wei-Yi He,
- Wei-Yi He,
- Guang Yang,
- Guang Yang,
- Guang Yang,
- Guang Yang,
- Min-Sheng You,
- Min-Sheng You,
- Min-Sheng You,
- Min-Sheng You
Affiliations
- Lu Peng
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Lu Peng
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Lu Peng
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Lu Peng
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Qing Wang
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Qing Wang
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Qing Wang
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Qing Wang
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Ming-Min Zou
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Ming-Min Zou
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Ming-Min Zou
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Ming-Min Zou
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Yu-Dong Qin
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Yu-Dong Qin
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Yu-Dong Qin
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Yu-Dong Qin
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Liette Vasseur
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Liette Vasseur
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Liette Vasseur
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Liette Vasseur
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Liette Vasseur
- Department of Biological Sciences, Brock University, St. Catharines, ON, Canada
- Li-Na Chu
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Li-Na Chu
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Li-Na Chu
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Li-Na Chu
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Yi-Long Zhai
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Yi-Long Zhai
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Yi-Long Zhai
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Yi-Long Zhai
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Shi-Jie Dong
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Shi-Jie Dong
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Shi-Jie Dong
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Shi-Jie Dong
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Li-Li Liu
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Li-Li Liu
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Li-Li Liu
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Li-Li Liu
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Wei-Yi He
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Wei-Yi He
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Wei-Yi He
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Wei-Yi He
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Guang Yang
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Guang Yang
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Guang Yang
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Guang Yang
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Min-Sheng You
- State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China
- Min-Sheng You
- Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- Min-Sheng You
- Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, China
- Min-Sheng You
- Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou, China
- DOI
- https://doi.org/10.3389/fphys.2019.01585
- Journal volume & issue
-
Vol. 10
Abstract
The vitellogenin receptor (VgR) belongs to the low-density lipoprotein receptor (LDLR) gene superfamily and plays an indispensable role in Vg transport, yolk deposition, and oocyte development. For this reason, it has become a promising target for pest control. The involvement of VgR in Vg transport and reproductive functions remains unclear in diamondback moths, Plutella xylostella (L.), a destructive pest of cruciferous crops. Here, we cloned and identified the complete cDNA sequence of P. xylostella VgR, which encoded 1805 amino acid residues and contained four conserved domains of LDLR superfamily. PxVgR was mainly expressed in female adults, more specifically in the ovary. PxVgR protein also showed the similar expression profile with the PxVgR transcript. CRISPR/Cas9-mediated PxVgR knockout created a homozygous mutant of P. xylostella with 5-bp-nucleotide deletion in the PxVgR. The expression deficiency of PxVgR protein was detected in the ovaries and eggs of mutant individuals. Vg protein was still detected in the eggs of the mutant individuals, but with a decreased expression level. However, PxVg transcripts were not significantly affected by the PxVgR knockout. Knockout of PxVgR resulted in shorter ovarioles of newly emerged females. No significant difference was detected between wild and mutant individuals in terms of the number of eggs laid in the first 3 days after mating. The loss of PxVgR gene resulted in smaller and whiter eggs and lower egg hatching rate. This study represents the first report on the functions of VgR in Vg transport, ovary development, oviposition, and embryonic development of P. xylostella using CRISPR/Cas9 technology. This study lays the foundation for understanding molecular mechanisms of P. xylostella reproduction, and for making use of VgR as a potential genetic-based molecular target for better control of the P. xylostella.
Keywords
- diamondback moth
- mutant lines
- CRISPR/Cas9
- Vg transport
- embryonic development
- reproductive regulation
