Flow Cytometry and T-Cell Response Monitoring after Smallpox Vaccination

Abstract

Orthopoxvirus zoonosis or smallpox as result of bioterrorism or biological warfare represents a risk for epidemic spread. By monitoring T-cell responses by flow cytometry, we observed a recall response after recent vaccination against smallpox. When the high similarity between the orthopoxviruses is considered, this rapid assay that uses vaccinia antigens could identify recently exposures.

Keywords

• antigen
• camelpox
• CMV
• cowpox
• cytokines
• cytomegalovirus