Prolonged DNA hydrolysis in water: A study on DNA stability

Paolo Fattorini; Giorgio Marrubini; Serena Bonin; Barbara Bertoglio; Pierangela Grignani; Elisa Recchia; Paola Pitacco; Francesca Procopio; Carolina Cantoni; Irena Zupanič Pajnič; Solange Sorçaburu-Cigliero; Carlo Previdere`

Data in Brief (Oct 2018)

Prolonged DNA hydrolysis in water: A study on DNA stability

Paolo Fattorini,
Giorgio Marrubini,
Serena Bonin,
Barbara Bertoglio,
Pierangela Grignani,
Elisa Recchia,
Paola Pitacco,
Francesca Procopio,
Carolina Cantoni,
Irena Zupanič Pajnič,
Solange Sorçaburu-Cigliero,
Carlo Previdere`

Affiliations

Paolo Fattorini: Department of Medicine, Surgery and Health, University of Trieste, Italy; Corresponding author. Fax: +39 040 3996265.
Giorgio Marrubini: Department of Drug Sciences, University of Pavia, Italy
Serena Bonin: Department of Medicine, Surgery and Health, University of Trieste, Italy
Barbara Bertoglio: Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy
Pierangela Grignani: Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy
Elisa Recchia: Department of Medicine, Surgery and Health, University of Trieste, Italy
Paola Pitacco: Department of Medicine, Surgery and Health, University of Trieste, Italy
Francesca Procopio: School of Biological and Chemical Sciences, Queen Mary University of London, United Kingdom
Carolina Cantoni: CNR-ISMAR, Trieste, Italy
Irena Zupanič Pajnič: Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Slovenia
Solange Sorçaburu-Cigliero: Department of Medicine, Surgery and Health, University of Trieste, Italy
Carlo Previdere`: Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy

Journal volume & issue: Vol. 20
pp. 1237 – 1243

Abstract

Read online

This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study. Keywords: qPCR, Degraded samples, DNA quantification, DNA hydrolysis

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

About the journal