Biology Open (Sep 2024)
Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase
- Junyan Ma,
- Weiting Zhang,
- Simin Rahimialiabadi,
- Nikkitha Umesh Ganesh,
- Zhengwang Sun,
- Saba Parvez,
- Randall T. Peterson,
- Jing-Ruey Joanna Yeh
Affiliations
- Junyan Ma
- Department of Basic Medical Science, Quanzhou Medical College, Quanzhou, Fujian 362011, China
- Weiting Zhang
- Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
- Simin Rahimialiabadi
- Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, UT 84112, USA
- Nikkitha Umesh Ganesh
- Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
- Zhengwang Sun
- Center for Immunology and Inflammatory Disease, Massachusetts General Hospital, Charlestown, MA 02129, USA
- Saba Parvez
- Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, UT 84112, USA
- Randall T. Peterson
- Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, UT 84112, USA
- Jing-Ruey Joanna Yeh
- Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
- DOI
- https://doi.org/10.1242/bio.061666
- Journal volume & issue
-
Vol. 13,
no. 9
Abstract
No abstracts available.Keywords
- crispr
- cas9
- phic31
- integrase
- targeted integration
- genotyping
- transgenesis
- reporter
- zebrafish
- knock-in