First Immunoassay for Measuring Isoaspartate in Human Serum Albumin

Jijing Wang; Susanna L. Lundström; Sven Seelow; Sergey Rodin; Zhaowei Meng; Juan Astorga-Wells; Qinyu Jia; Roman A. Zubarev

doi:10.3390/molecules26216709

Molecules (Nov 2021)

First Immunoassay for Measuring Isoaspartate in Human Serum Albumin

Jijing Wang,
Susanna L. Lundström,
Sven Seelow,
Sergey Rodin,
Zhaowei Meng,
Juan Astorga-Wells,
Qinyu Jia,
Roman A. Zubarev

Affiliations

Jijing Wang: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden
Susanna L. Lundström: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden
Sven Seelow: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden
Sergey Rodin: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden
Zhaowei Meng: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden
Juan Astorga-Wells: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden
Qinyu Jia: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden
Roman A. Zubarev: Department of Medical Biophysics and Biochemistry, Karolinska Institute, 171 77 Stockholm, Sweden

DOI: https://doi.org/10.3390/molecules26216709
Journal volume & issue: Vol. 26, no. 21
p. 6709

Abstract

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Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer’s disease (AD). Here we report on a novel monoclonal antibody (mAb) 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA. Based on 1A3 mAb, an indirect enzyme-linked immunosorbent assay (ELISA) was developed, and the isoAsp occupancy in 100 healthy plasma samples was quantified for the first time, providing the average value of (0.74 ± 0.13)%. These results suggest potential of isoAsp measurements for supplementary AD diagnostics as well as for assessing the freshness of stored donor blood and its suitability for transfusion.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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