Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T

Thomas Nerreter; Sebastian Letschert; Ralph Götz; Sören Doose; Sophia Danhof; Hermann Einsele; Markus Sauer; Michael Hudecek

doi:10.1038/s41467-019-10948-w

Nature Communications (Jul 2019)

Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T

Thomas Nerreter,
Sebastian Letschert,
Ralph Götz,
Sören Doose,
Sophia Danhof,
Hermann Einsele,
Markus Sauer,
Michael Hudecek

Affiliations

Thomas Nerreter: Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg
Sebastian Letschert: Department of Biotechnology and Biophysics, Biocenter, Julius Maximilian University Würzburg, Am Hubland
Ralph Götz: Department of Biotechnology and Biophysics, Biocenter, Julius Maximilian University Würzburg, Am Hubland
Sören Doose: Department of Biotechnology and Biophysics, Biocenter, Julius Maximilian University Würzburg, Am Hubland
Sophia Danhof: Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg
Hermann Einsele: Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg
Markus Sauer: Department of Biotechnology and Biophysics, Biocenter, Julius Maximilian University Würzburg, Am Hubland
Michael Hudecek: Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg

DOI: https://doi.org/10.1038/s41467-019-10948-w
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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CD19 CAR-T cells have achieved some success in treating myeloma patients despite the limited detection of the CD19 antigen. Here, the authors show using dSTORM that 10/14 myeloma samples studied express ultra-low levels of CD19, which are sufficient for engaging CAR-T cells in vitro.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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