PLoS ONE (Jan 2021)

WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva.

  • David Ng,
  • Ana Pinharanda,
  • Merly C Vogt,
  • Ashok Litwin-Kumar,
  • Kyle Stearns,
  • Urvashi Thopte,
  • Enrico Cannavo,
  • Armen Enikolopov,
  • Felix Fiederling,
  • Stylianos Kosmidis,
  • Barbara Noro,
  • Ines Rodrigues-Vaz,
  • Hani Shayya,
  • Peter Andolfatto,
  • Darcy S Peterka,
  • Tanya Tabachnik,
  • Jeanine D'Armiento,
  • Monica Goldklang,
  • Andres Bendesky

DOI
https://doi.org/10.1371/journal.pone.0257464
Journal volume & issue
Vol. 16, no. 9
p. e0257464

Abstract

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Despite the development of effective vaccines against SARS-CoV-2, epidemiological control of the virus is still challenging due to slow vaccine rollouts, incomplete vaccine protection to current and emerging variants, and unwillingness to get vaccinated. Therefore, frequent testing of individuals to identify early SARS-CoV-2 infections, contact-tracing and isolation strategies remain crucial to mitigate viral spread. Here, we describe WHotLAMP, a rapid molecular test to detect SARS-CoV-2 in saliva. WHotLAMP is simple to use, highly sensitive (~4 viral particles per microliter of saliva) and specific, as well as inexpensive, making it ideal for frequent screening. Moreover, WHotLAMP does not require toxic chemicals or specialized equipment and thus can be performed in point-of-care settings, and may also be adapted for resource-limited environments or home use. While applied here to SARS-CoV-2, WHotLAMP can be modified to detect other pathogens, making it adaptable for other diagnostic assays, including for use in future outbreaks.