Scientific Reports (Jun 2021)
Serum anti-AP3D1 antibodies are risk factors for acute ischemic stroke related with atherosclerosis
- Shu-Yang Li,
- Yoichi Yoshida,
- Eiichi Kobayashi,
- Masaaki Kubota,
- Tomoo Matsutani,
- Seiichiro Mine,
- Toshio Machida,
- Yoshiro Maezawa,
- Minoru Takemoto,
- Koutaro Yokote,
- Yoshio Kobayashi,
- Hirotaka Takizawa,
- Mizuki Sata,
- Kazumasa Yamagishi,
- Hiroyasu Iso,
- Norie Sawada,
- Shoichiro Tsugane,
- Sohei Kobayashi,
- Kazuyuki Matsushita,
- Fumio Nomura,
- Hisahiro Matsubara,
- Makoto Sumazaki,
- Masaaki Ito,
- Satoshi Yajima,
- Hideaki Shimada,
- Katsuro Iwase,
- Hiromi Ashino,
- Hao Wang,
- Kenichiro Goto,
- Go Tomiyoshi,
- Natsuko Shinmen,
- Rika Nakamura,
- Hideyuki Kuroda,
- Yasuo Iwadate,
- Takaki Hiwasa
Affiliations
- Shu-Yang Li
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Yoichi Yoshida
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Eiichi Kobayashi
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Masaaki Kubota
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Tomoo Matsutani
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Seiichiro Mine
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Toshio Machida
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Yoshiro Maezawa
- Department of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University
- Minoru Takemoto
- Department of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University
- Koutaro Yokote
- Department of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University
- Yoshio Kobayashi
- Department of Cardiovascular Medicine, Graduate School of Medicine, Chiba University
- Hirotaka Takizawa
- Port Square Kashiwado Clinic, Kashiwado Memorial Foundation
- Mizuki Sata
- Department of Public Health Medicine, Faculty of Medicine, University of Tsukuba
- Kazumasa Yamagishi
- Department of Public Health Medicine, Faculty of Medicine, University of Tsukuba
- Hiroyasu Iso
- Department of Public Health, Social Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University
- Norie Sawada
- Epidemiology and Prevention Group, Center for Public Health Sciences, National Cancer Center
- Shoichiro Tsugane
- Epidemiology and Prevention Group, Center for Public Health Sciences, National Cancer Center
- Sohei Kobayashi
- Department of Laboratory Medicine and Division of Clinical Genetics, Chiba University Hospital
- Kazuyuki Matsushita
- Department of Laboratory Medicine and Division of Clinical Genetics, Chiba University Hospital
- Fumio Nomura
- Division of Clinical Genetics, Chiba Foundation for Health Promotion & Disease Prevention
- Hisahiro Matsubara
- Department of Frontier Surgery, Graduate School of Medicine, Chiba University
- Makoto Sumazaki
- Department of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine
- Masaaki Ito
- Department of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine
- Satoshi Yajima
- Department of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine
- Hideaki Shimada
- Department of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine
- Katsuro Iwase
- Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University
- Hiromi Ashino
- Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University
- Hao Wang
- Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University
- Kenichiro Goto
- Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University
- Go Tomiyoshi
- Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University
- Natsuko Shinmen
- Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University
- Rika Nakamura
- Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University
- Hideyuki Kuroda
- Medical Project Division, Research Development Center, Fujikura Kasei Co.
- Yasuo Iwadate
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- Takaki Hiwasa
- Department of Neurological Surgery, Graduate School of Medicine, Chiba University
- DOI
- https://doi.org/10.1038/s41598-021-92786-9
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 15
Abstract
Abstract Atherosclerosis has been considered as the main cause of morbidity, mortality, and disability worldwide. The first screening for antigen markers was conducted using the serological identification of antigens by recombinant cDNA expression cloning, which has identified adaptor-related protein complex 3 subunit delta 1 (AP3D1) as an antigen recognized by serum IgG antibodies of patients with atherosclerosis. Serum antibody levels were examined using the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) using a recombinant protein as an antigen. It was determined that the serum antibody levels against AP3D1 were higher in patients with acute ischemic stroke (AIS), transient ischemic attack, diabetes mellitus (DM), cardiovascular disease, chronic kidney disease (CKD), esophageal squamous cell carcinoma (ESCC), and colorectal carcinoma than those in the healthy donors. The area under the curve values of DM, nephrosclerosis type of CKD, and ESCC calculated using receiver operating characteristic curve analysis were higher than those of other diseases. Correlation analysis showed that the anti-AP3D1 antibody levels were highly associated with maximum intima-media thickness, which indicates that this marker reflected the development of atherosclerosis. The results of the Japan Public Health Center-based Prospective Study indicated that this antibody marker is deemed useful as risk factors for AIS.
