Plant regeneration from excised bulb scale segments of Zephyranthes robusta Baker

Abstract

Zephyranthes robusta Baker excised bulb scale segments with the basal plate were grown on Murashige and Skoog (MS) medium in four modifications. The cultures were kept at 25°C in darkness. The best results (bulbing, leaf development and rooting) were obtained on MS medium with 1 mg/l IBA, 2-6 bulblets being developed from one explant. After 6 weeks the plantlets (4-6 cm high) were transferred to pots filled with sterilized soil mixes. After two months the leaves of the plants reached a length of 20 cm.