Frontiers in Immunology (Feb 2021)
In Nasal Mucosal Secretions, Distinct IFN and IgA Responses Are Found in Severe and Mild SARS-CoV-2 Infection
- Juliana de Melo Batista dos Santos,
- Camila Pereira Soares,
- Fernanda Rodrigues Monteiro,
- Fernanda Rodrigues Monteiro,
- Ralyria Mello,
- Jonatas Bussador do Amaral,
- Andressa Simões Aguiar,
- Andressa Simões Aguiar,
- Mariana Pereira Soledade,
- Carolina Sucupira,
- Milena De Paulis,
- Juliana Bannwart Andrade,
- Flavia Jaqueline Almeida,
- Marco Aurélio Palazzi Sáfadi,
- Luciana Becker Mau,
- Jamile Menezes Brasil,
- Theresa Ramalho,
- Flávio V. Loures,
- Rodolfo Paula Vieira,
- Rodolfo Paula Vieira,
- Rodolfo Paula Vieira,
- Edison Luiz Durigon,
- Edison Luiz Durigon,
- Danielle Bruna Leal de Oliveira,
- Danielle Bruna Leal de Oliveira,
- André Luis Lacerda Bachi,
- André Luis Lacerda Bachi
Affiliations
- Juliana de Melo Batista dos Santos
- Post-graduation Program in Sciences of Human Movement and Rehabilitation, Federal University of São Paulo, São Paulo, Brazil
- Camila Pereira Soares
- Laboratory of Clinical and Molecular Virology, Department of Microbiology, Institute of Biomedical Science of University of São Paulo, São Paulo, Brazil
- Fernanda Rodrigues Monteiro
- Ear, Nose and Throat (ENT) Lab, Department of Otorhinolaryngology, Federal University of São Paulo, São Paulo, Brazil
- Fernanda Rodrigues Monteiro
- Method Faculty of São Paulo, São Paulo, Brazil
- Ralyria Mello
- Laboratory of Clinical and Molecular Virology, Department of Microbiology, Institute of Biomedical Science of University of São Paulo, São Paulo, Brazil
- Jonatas Bussador do Amaral
- Ear, Nose and Throat (ENT) Lab, Department of Otorhinolaryngology, Federal University of São Paulo, São Paulo, Brazil
- Andressa Simões Aguiar
- Infection Control Service, São Luiz Gonzaga Hospital of Santa Casa de Misericordia os São Paulo, São Paulo, Brazil
- Andressa Simões Aguiar
- Infection Control Service and Epidemiological Hospital Nucleo, Municipal Children's Hospital Candido Fontoura, São Paulo, Brazil
- Mariana Pereira Soledade
- Infection Control Service and Epidemiological Hospital Nucleo, Municipal Children's Hospital Candido Fontoura, São Paulo, Brazil
- Carolina Sucupira
- Infection Control Service and Epidemiological Hospital Nucleo, Municipal Children's Hospital Candido Fontoura, São Paulo, Brazil
- Milena De Paulis
- Department of Pediatrics, School of Medicine, University Hospital, University of São Paulo, São Paulo, Brazil
- Juliana Bannwart Andrade
- Department of Pediatrics, School of Medicine, University Hospital, University of São Paulo, São Paulo, Brazil
- Flavia Jaqueline Almeida
- Department of Pediatrics, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil
- Marco Aurélio Palazzi Sáfadi
- Department of Pediatrics, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil
- Luciana Becker Mau
- Infection Control Service and Epidemiological Hospital Nucleo, Menino Jesus Municipal Hospital, São Paulo, Brazil
- Jamile Menezes Brasil
- Infection Control Service and Epidemiological Hospital Nucleo, Menino Jesus Municipal Hospital, São Paulo, Brazil
- Theresa Ramalho
- 0Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
- Flávio V. Loures
- 1Institute of Science and Technology, Federal University of São Paulo, São Paulo, Brazil
- Rodolfo Paula Vieira
- Post-graduation Program in Sciences of Human Movement and Rehabilitation, Federal University of São Paulo, São Paulo, Brazil
- Rodolfo Paula Vieira
- 2Post-graduation Program in Bioengineering and Biomedical Engineering, Universidade Brasil, São Paulo, Brazil
- Rodolfo Paula Vieira
- 3Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), Sao Jose dos Campos, Brazil
- Edison Luiz Durigon
- Laboratory of Clinical and Molecular Virology, Department of Microbiology, Institute of Biomedical Science of University of São Paulo, São Paulo, Brazil
- Edison Luiz Durigon
- 4Scientific Platform Pasteur University of São Paulo, São Paulo, Brazil
- Danielle Bruna Leal de Oliveira
- Laboratory of Clinical and Molecular Virology, Department of Microbiology, Institute of Biomedical Science of University of São Paulo, São Paulo, Brazil
- Danielle Bruna Leal de Oliveira
- 4Scientific Platform Pasteur University of São Paulo, São Paulo, Brazil
- André Luis Lacerda Bachi
- Ear, Nose and Throat (ENT) Lab, Department of Otorhinolaryngology, Federal University of São Paulo, São Paulo, Brazil
- André Luis Lacerda Bachi
- 5Post-graduation Program in Health Science, University of Santo Amaro, São Paulo, Brazil
- DOI
- https://doi.org/10.3389/fimmu.2021.595343
- Journal volume & issue
-
Vol. 12
Abstract
Likely as in other viral respiratory diseases, SARS-CoV-2 elicit a local immune response, which includes production and releasing of both cytokines and secretory immunoglobulin (SIgA). Therefore, in this study, we investigated the levels of specific-SIgA for SARS-CoV-2 and cytokines in the airways mucosa 37 patients who were suspected of COVID-19. According to the RT-PCR results, the patients were separated into three groups: negative for COVID-19 and other viruses (NEGS, n = 5); negative for COVID-19 but positive for the presence of other viruses (OTHERS, n = 5); and the positive for COVID-19 (COVID-19, n = 27). Higher specific-SIgA for SARS-CoV-2, IFN-β, and IFN-γ were found in the COVID-19 group than in the other groups. Increased IL-12p70 levels were observed in OTHERS group as compared to COVID-19 group. When the COVID-19 group was sub stratified according to the illness severity, significant differences and correlations were found for the same parameters described above comparing severe COVID-19 to the mild COVID-19 group and other non-COVID-19 groups. For the first time, significant differences are shown in the airway's mucosa immune responses in different groups of patients with or without respiratory SARS-CoV-2 infection.
Keywords
- SARS-CoV-2
- cytokine-immunological terms
- respiratory
- viruses
- mucosal immmunity
- secretory immunoglobulin A
