Overexpression of transcriptional regulator and tailoring enzyme leads to the discovery of anti-inflammatory meroterpenoids from marine-derived fungus Alternaria alternata JJY-32

Guowei Liu; Dan Liu; Zongyuan Li; Jieying Jiao; Xuewen Hou; Xiaomin Zhang; Qian Che; Tianjiao Zhu; Dehai Li; Dehai Li; Guojian Zhang; Guojian Zhang; Guojian Zhang

doi:10.3389/fmars.2022.1015684

Frontiers in Marine Science (Oct 2022)

Overexpression of transcriptional regulator and tailoring enzyme leads to the discovery of anti-inflammatory meroterpenoids from marine-derived fungus Alternaria alternata JJY-32

Guowei Liu,
Dan Liu,
Zongyuan Li,
Jieying Jiao,
Xuewen Hou,
Xiaomin Zhang,
Qian Che,
Tianjiao Zhu,
Dehai Li,
Dehai Li,
Guojian Zhang,
Guojian Zhang,
Guojian Zhang

Affiliations

Guowei Liu: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Dan Liu: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Zongyuan Li: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Jieying Jiao: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Xuewen Hou: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Xiaomin Zhang: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Qian Che: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Tianjiao Zhu: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Dehai Li: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Dehai Li: Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology, Qingdao, China
Guojian Zhang: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
Guojian Zhang: Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology, Qingdao, China
Guojian Zhang: Lab of Marine Medicinal Resources Discovery, Marine Biomedical Research Institute of Qingdao, Qingdao, China

DOI: https://doi.org/10.3389/fmars.2022.1015684
Journal volume & issue: Vol. 9

Abstract

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Four new ACTG-toxins like meroterpenoids tricycloalternarenes O-R were discovered from a marine-derived fungus Alternaria alternata JJY-32 by the overexpression of a LaeA-like global transcriptional regulator and a MpaB-like biosynthetic tailoring enzyme. Among those structures, tricycloalternarene O possesses a non-canonical cyclohexanone-pyran fused furan ring structure and tricycloalternarene R contains a truncated isoprenoid terminal. The structures were elucidated based on comprehensive 1D and 2D NMR data and assisted by ECD calculations. Tricycloalternarenes O-R showed potent anti-inflammatory activity in TLR4-transfected microphage cells (RAW264.7).

Published in Frontiers in Marine Science

ISSN: 2296-7745 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Natural history (General): General. Including nature conservation, geographical distribution
Website: https://www.frontiersin.org/journals/marine-science

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