Frontiers in Immunology (Oct 2023)

The safety and anti-tumor effect of multiple peptides-pulsed dendritic cells combined with induced specific cytotoxic T lymphocytes for patients with solid tumors

  • Xuan Zhao,
  • Xuan Zhao,
  • Xuan Zhao,
  • Zhen Zhang,
  • Zhen Zhang,
  • Zhen Zhang,
  • Chunli Wen,
  • Jianmin Huang,
  • Jianmin Huang,
  • Jianmin Huang,
  • Shuangning Yang,
  • Shuangning Yang,
  • Shuangning Yang,
  • Jinyan Liu,
  • Jinyan Liu,
  • Jinyan Liu,
  • Huizhen Geng,
  • Bing Peng,
  • Zibo Li,
  • Yi Zhang,
  • Yi Zhang,
  • Yi Zhang,
  • Yi Zhang

DOI
https://doi.org/10.3389/fimmu.2023.1284334
Journal volume & issue
Vol. 14

Abstract

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ObjectiveThe aim of this study was to explore the safety and efficacy of multiple peptide-pulsed autologous dendritic cells (DCs) combined with cytotoxic T lymphocytes (CTLs) in patients with cancer.MethodsFive patients diagnosed with cancer between November 2020 and June 2021 were enrolled and received DC-CTLs therapy. Peripheral blood was collected and antigenic peptides were analyzed. The phenotype and function of DC-CTLs and the immune status of patients were detected using flow cytometry or IFN-γ ELISPOT analysis.ResultsDCs acquired a mature phenotype and expressed high levels of CD80, CD86, CD83, and HLA-DR after co-culture with peptides, and the DC-CTLs also exhibited high levels of IFN-γ. Peripheral blood mononuclear cells from post-treatment patients showed a stronger immune response to peptides than those prior to treatment. Importantly, four of five patients maintained a favorable immune status, of which one patient’s disease-free survival lasted up to 28.2 months. No severe treatment-related adverse events were observed.ConclusionOur results show that multiple peptide-pulsed DCs combined with CTLs therapy has manageable safety and promising efficacy for cancer patients, which might provide a precise immunotherapeutic strategy for cancer.

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