Офтальмохирургия (Oct 2020)

Transforming growth factor TGF-β in various small choroid melanoma clinical characteristics before and after eye-preserving treatment

  • E. B. Myakoshina,
  • I. G. Kulikova,
  • N. V. Balatskaya,
  • L. A. Katargina,
  • S. V. Saakyan

DOI
https://doi.org/10.25276/0235-4160-2020-3-58-64
Journal volume & issue
Vol. 0, no. 3
pp. 58 – 64

Abstract

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Purpose. To study the role of transforming growth factor TGF-β in various small choroidal melanoma clinical characteristics before and after eye-preserving treatment.Material and methods. In total, the study included 111 people with an average age of 53.7±12.2 years with choroidal melanoma (n=78) and healthy donors (n=33). Group 1 (n=32) included patients with small choroidal melanoma; group 2 (n=25) included patients with medium-sized tumors; group 3 (n=21) included patients with large melanomas. All patients with choroid melanoma were treated by laser coagulation, transpupillary thermotherapy, brachytherapy, observation after 7±1.2months. A study of transforming growth factor (TGF-β1 and TGF-β2) was performed on a BD FACS Canto II flow cytometer. Statistical processing: Student’s t-test, Fisher criteria and χ2, p<0.05.Results. By analyzing the content of TGF-β1 and TGF-β2 in blood serum, significantly higher indices were noted in small choroidal melanoma with retinal exudate (19664.2±1708.7 and 4914.4±325.2 pkg/ml, respectively) compared with those without exudate (18778.7±1609.6 and 3857.3±275.1 pcg/ml, respectively). Comparing the dynamics of the content of TGF-β1 and TGF-β2 in blood serum in small stage of tumor (19902.2±1406 and 4220.7±277.8 pkg/ml, respectively) big tumor (23143.1±1984.5 and 4591.7±334.1 pcg/ml, respectively) we noted its significant increase with the growth of melanoma. The amount of TGF-β2 in the blood serum with bad course (presence of residual tumor or continued growth) of small choroidal melanoma after treatment was significantly higher (4864.1±1299.99 pcg / ml) compared with the good situation (3649.5±1216.5 pcg / ml).Conclusion. Our data may indicate the role of transforming growth factor (TGF-β) in the bad course of small choroidal melanoma and serve as an occasion for the development of targeted anti-TGF–β therapy in combination with radiation therapy.

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