Enhancing diabetic rat peripheral nerve conduction velocity parameters through the potent effects of MitoTEMPO

Abstract

The efficacy of MitoTEMPO, a mitochondria-targeted antioxidant, in altering nerve fiber conduction properties within the sciatic nerve of streptozotocin (STZ)-induced diabetic rats, a model for diabetic neuropathy characterized by myelinated fiber atrophy and nodal abnormalities. Utilizing the STZ-induced diabetic rat model, we assessed the impact of MitoTEMPO on nerve function through compound action potential (CAP) analysis and histological evaluation. Key indicators such as maximum depolarization (MD), CAP area, and conduction velocity distribution (CVD) were measured to gauge MitoTEMPO's neuroprotective effects, alongside physical parameters like weight and blood glucose levels. MitoTEMPO treatment significantly preserved CAP parameters (MD and CAP area), indicative of its protective role against diabetes-induced nerve dysfunction. Moreover, it mitigated the diabetes-induced disruption in CVD, suggesting a preservation of nerve fiber activity. Histological analyses corroborated these electrophysiological findings, showing reduced damage in MitoTEMPO-treated nerves, such as thinner perineurium and less myelin sheath degeneration. Our findings highlight MitoTEMPO's potential as a mitigative agent against diabetic neuropathy's detrimental effects on nerve structure and function. The study underscores the therapeutic promise of mitochondria-specific antioxidants in managing diabetic neuropathy, advocating for further clinical research to explore MitoTEMPO's applicability in novel treatment strategies for peripheral neuropathies.

Keywords

• MitoTEMPO
• Nerve conduction velocity studies
• Diabetic neuropathy
• Streptozotocin