Determining the metabolic impact of postbiotics in mice

Fernando F. Anhê; Arshpreet Bhatwa; Jonathan D. Schertzer

STAR Protocols (Mar 2022)

Determining the metabolic impact of postbiotics in mice

Fernando F. Anhê,
Arshpreet Bhatwa,
Jonathan D. Schertzer

Affiliations

Fernando F. Anhê: Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Farncombe Family Digestive Health Research Institute, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Centre for Metabolism, Obesity and Diabetes Research, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Corresponding author
Arshpreet Bhatwa: Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Farncombe Family Digestive Health Research Institute, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Centre for Metabolism, Obesity and Diabetes Research, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada
Jonathan D. Schertzer: Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Farncombe Family Digestive Health Research Institute, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Centre for Metabolism, Obesity and Diabetes Research, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada; Corresponding author

Journal volume & issue: Vol. 3, no. 1
p. 101098

Abstract

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Summary: Postbiotics cooperate to influence immune and metabolic outcomes in the host. Here we describe a protocol for in vivo assessment of blood glucose control following acute administration of lipopolysaccharide (LPS) and peptidoglycan (PGN) in mice. This protocol can be adapted for testing a broad range of microbial molecules and ligands for host immune receptors. Experience with mouse handling is required.For complete details on the use and execution of this protocol, please refer to Anhê et al. (2021) and Cavallari et al. (2017).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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