Pengukuran Konsentrasi dan Kemurnian DNA Genom Nyamuk Anopheles barbirostris

Hasrida Mustafa; Indra Rachmawati; Yusran Udin

doi:10.22435/vektorp.v10i1.6251.7-10

Jurnal Vektor Penyakit (Feb 2017)

Pengukuran Konsentrasi dan Kemurnian DNA Genom Nyamuk Anopheles barbirostris

Hasrida Mustafa,
Indra Rachmawati,
Yusran Udin

Affiliations

Hasrida Mustafa: Balai Litbang P2B2 Donggala, Badan Litbang Kesehatan, Kementerian Kesehatan RI, Jl. Masitudju No.58 Labuan Panimba, Labuan, Donggala, Sulawesi Tengah, Indonesia
Indra Rachmawati: Badan Pengkajian dan Penerapan Teknologi (BPPT), Jalan MH.Thamrin 8, Jakarta 10340
Yusran Udin: Balai Litbang P2B2 Donggala, Badan Litbang Kesehatan, Kementerian Kesehatan RI, Jl. Masitudju No.58 Labuan Panimba, Labuan, Donggala, Sulawesi Tengah, Indonesia

DOI: https://doi.org/10.22435/vektorp.v10i1.6251.7-10
Journal volume & issue: Vol. 10, no. 1
pp. 7 – 10

Abstract

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The purity and concentration of DNA information from some sample are important to measure the degree of contamination. Anopheles barbirostris mosquitoes samples from Kalukutinggu Village was extracted to obtain the genome DNA using PureLink Genomic DNA mini Kits. The purity and concentration of DNA Anopheles barbirostris was measured by using nano spectrophotometer. The results showed that DNA concentration was 17,33 – 34,79 ug/ml, and their purity were 1,90 average. The purity and concentration of the DNA from the samples showed were not contaminated and eligible for the next step, that is DNA amplification

Published in Jurnal Vektor Penyakit

ISSN: 1978-3647 (Print); 2354-8835 (Online)
Publisher: Badan Penelitian dan Pengembangan Kesehatan
Country of publisher: Indonesia
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: http://ejournal2.litbang.kemkes.go.id/index.php/vektorp/index

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