Antibacterial Activity of Green Synthesised Silver Nanoparticles on <i>Saccharomyces cerevisiae</i>

Abstract

Green synthesis of nanoparticles is a widely researched and popular direction in the development of nanotechnology. It is a simple, cheap and effective method for obtaining nanoparticles with interesting biological properties. In light of the development of antibiotic resistance to important clinical strains of bacteria, this method was used in the present study to obtain silver nanoparticles with antibacterial activity. The aim of this study was to synthesise silver nanoparticles with antibacterial action by yeast in a process known as “green synthesis”. We are also considering the prospect of using silver nanoparticles as an antibacterial substance for drug development. The production of nanoparticles was confirmed by UV spectroscopy. Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 test strains and Staphylococcus aureus 1536 and Klebsiella pneumoniae 520 clinical isolates were used to study the antibacterial effect. The effect of synthesised nanoparticles on the metabolic activity of bacterial cells and their ability to adhere, as well as the minimum inhibitory concentrations (MICs) of synthesised nanoparticles for each of the strains, were determined. Following UV spectroscopy, the nanoparticles obtained were found to have a pronounced peak in optical absorption at 400 nm, corresponding to the plasmon resonance of silver nanoparticles, and demonstrated a high antibacterial effect against all the strains studied.

Keywords

• silver nanoparticles
• <i>Saccharomyces cerevisiae</i>
• antibacterial activity
• clinical isolates
• <i>Staphylococcus aureus</i>
• <i>Klebsiella pneumoniae</i>