PeerJ (Apr 2024)
Assessing the impact of two conventional wastewater treatment plants on small streams with effect-based methods
Abstract
Sixty percent of discrete surface water bodies in Europe do not meet the requirements for good ecological and chemical status and in Germany, the situation is even worse with over 90% of surface water bodies failing to meet the threshold. In addition to hydromorphological degradation, intensive land use and invasive species, chemical pollution is primarily considered to be responsible for the inadequate ecological status of the water bodies. As a quantitatively important source of micropollutants, wastewater treatment plants (WWTPs) represent an important entry path for chemical stressors. It is therefore important to analyze the effectiveness of the WWTPs in eliminating micropollutants and other chemical stressors to mitigate the negative impacts of the treated wastewater (WW) in aquatic ecosystems. Accordingly, in this study, we evaluated the impacts of two conventional, medium-sized WWTPs on their small receiving water systems in the southwestern region of Hessen in Germany during two sampling campaigns (spring and fall) using effect-based methods (EBM). We hypothesized that due to the insufficient elimination of micropollutants, a broad spectrum of toxic effects would be detected in conventionally treated WW and also in the receiving surface waters downstream the WWTPs. As EBMs a battery of in vitro assays and active biomonitoring using two in vivo assays were applied. The results supported our hypothesis and showed that the untreated WW had a very high baseline toxicity and also high endocrine and mutagenic activities. Conventional WW treatment, consisting of mechanical and biological treatment with nitrification, denitrification and phosphate precipitation, reduced baseline toxicity by more than 90% and endocrine activities by more than 80% in both WWTPs. Despite these high elimination rates, the remaining baseline toxicity, the endocrine, dioxin-like and mutagenic activities of the conventionally treated WW were so high that negative effects on the two receiving waters were to be expected. This was confirmed in the active monitoring with the amphipod Gammarus fossarum and the mudsnail Potamopyrgus antipodarum, as mortality of both species increased downstream of the WWTPs and reproduction in P. antipodarum was also affected. These results indicate that advanced WW treatment is needed to more effectively eliminate chemical stressors to prevent negative impacts of treated WW particularly in small receiving waters.
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