Insecticidal investigation of Berberis Royleana various extracts against Callosobruchus Maculatus

Abstract

B. royleana is a rare species among Berberis, their root’s antiperiodic, antipyretic, and diaphoretic activity was said to be comparable to quinine. The shoot has antibacterial, anti-periodic, cardiovascular, hepatoprotective, and anti-cancer properties. In this study, Berberis royleana plant methanolic, n-hexane, chloroform, water, and ethyl acetate extracts were prepared and their in vitro insecticidal potential was explored against insect Callosobruchus maculatus. The 100µg of each extract was tested by direct contact application process at three different time (24, 48, 72 hours) intervals. The maximum mortality rate of 60% was recorded by water fraction, the methanolic fraction has 50% mortality, n-Hexane has 40% mortality, while ethyl acetate and chloroform have 30% mortality at 72 hours. This study concluded that the medicinal point of view B. royleana is very important and it has potential insecticidal activities against C. maculatus. This is the first study in Pakistan regarding insecticidal effects of B. royleana extracts against C. maculatus and may be further investigated against dengue and malarial vectors in the current pandemic situation in Pakistan.

Keywords

• Berberis royleana
• Callosobruchus maculatus
• insecticidal
• Pakistan